Waste liquid from other people's laboratories...
Today's protagonist is the beloved luminous substance luminol. This version looks like a mini flamethrower underwater, but it doesn't involve combustion. In essence, luminol molecules are oxidized to emit blue light:
the original video recorder of this image is Dr. Kendra Frederick (Twitter: @ kendrakf13). Interestingly, this is not a specially designed demonstration experiment, but a small idea arising from the treatment of experimental waste liquid. The solution in the beaker is 10% sodium hypochlorite bleach (acts as an oxidizer), and the needle is injected with the remaining chemiluminescence reagent containing luminol. When luminol is oxidized by an appropriate oxidant under alkaline conditions, an "excited" intermediate is produced, and when the electrons in it return to the ground state, they emit blue light.
the luminol reagent here was originally used to detect proteins. In Western blotting (western blot), antibodies linked to the enzyme are often used to bind to the protein to be detected, while luminol chemiluminescence reagent combined with the enzyme (peroxidase) can cause local luminescence and show the position of the protein. The luminescence in the picture is very bright, probably because the chemiluminescence enhancer is added to the reagent. Of course, when the protein is actually detected, the glow is far less bright.
this experiment has received a lot of praise on Twitter and reddit, and I didn't expect it to be so flaming.
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more luminol reaction (play is very varied):
dynamic picture appreciation: glowing fountain
dynamic picture appreciation: lighting copper wire
Luminol reaction, but also can make blue light flashing version